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FIG. 1. Screen of 33B8 binding deficient PAI-1 mutants. Panels A–C, filter series showing EX(lox) plaques. Panel A, plaques immunore- active with murine monoclonal 33B8; panel B, plaques reactive with rabbit anti-PAI-1 polyclonal antibodies; panel C, a merged image of A and B. The same filter is shown in each case. The dark spots indicate PAI-1antibody complexes, and the circles indicate examples of PAI-1 mutants that are specifically disrupted in 33B8 binding. Panels D and E show immunoblots of cell-free extracts of wild-type and parental PAI-1 mutants selected from the screen. Panel D was probed with 33B8 and panel E with anti-PAI-1 polyclonal antibodies. Panel F shows the inhibitory activity toward <t>high</t> <t>molecular</t> weight <t>uPA</t> of wild-type and each of the PAI-1 parental mutants in cell-free extracts. The inhibitory activity is expressed as the percentage of active PAI-1 protein relative to the total soluble protein of the cell extracts.
Upa, supplied by Innovative Research Inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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FIG. 1. Screen of 33B8 binding deficient PAI-1 mutants. Panels A–C, filter series showing EX(lox) plaques. Panel A, plaques immunore- active with murine monoclonal 33B8; panel B, plaques reactive with rabbit anti-PAI-1 polyclonal antibodies; panel C, a merged image of A and B. The same filter is shown in each case. The dark spots indicate PAI-1antibody complexes, and the circles indicate examples of PAI-1 mutants that are specifically disrupted in 33B8 binding. Panels D and E show immunoblots of cell-free extracts of wild-type and parental PAI-1 mutants selected from the screen. Panel D was probed with 33B8 and panel E with anti-PAI-1 polyclonal antibodies. Panel F shows the inhibitory activity toward <t>high</t> <t>molecular</t> weight <t>uPA</t> of wild-type and each of the PAI-1 parental mutants in cell-free extracts. The inhibitory activity is expressed as the percentage of active PAI-1 protein relative to the total soluble protein of the cell extracts.
Rabbit Anit Human Upa Affinity Purified Antibody, supplied by Innovative Research Inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Technoclone gmbh polyclonal antibodies against human u-pa
FIG. 1. Screen of 33B8 binding deficient PAI-1 mutants. Panels A–C, filter series showing EX(lox) plaques. Panel A, plaques immunore- active with murine monoclonal 33B8; panel B, plaques reactive with rabbit anti-PAI-1 polyclonal antibodies; panel C, a merged image of A and B. The same filter is shown in each case. The dark spots indicate PAI-1antibody complexes, and the circles indicate examples of PAI-1 mutants that are specifically disrupted in 33B8 binding. Panels D and E show immunoblots of cell-free extracts of wild-type and parental PAI-1 mutants selected from the screen. Panel D was probed with 33B8 and panel E with anti-PAI-1 polyclonal antibodies. Panel F shows the inhibitory activity toward <t>high</t> <t>molecular</t> weight <t>uPA</t> of wild-type and each of the PAI-1 parental mutants in cell-free extracts. The inhibitory activity is expressed as the percentage of active PAI-1 protein relative to the total soluble protein of the cell extracts.
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American Diagnostica rabbit anti-human upa antibody 389
FIG. 1. Screen of 33B8 binding deficient PAI-1 mutants. Panels A–C, filter series showing EX(lox) plaques. Panel A, plaques immunore- active with murine monoclonal 33B8; panel B, plaques reactive with rabbit anti-PAI-1 polyclonal antibodies; panel C, a merged image of A and B. The same filter is shown in each case. The dark spots indicate PAI-1antibody complexes, and the circles indicate examples of PAI-1 mutants that are specifically disrupted in 33B8 binding. Panels D and E show immunoblots of cell-free extracts of wild-type and parental PAI-1 mutants selected from the screen. Panel D was probed with 33B8 and panel E with anti-PAI-1 polyclonal antibodies. Panel F shows the inhibitory activity toward <t>high</t> <t>molecular</t> weight <t>uPA</t> of wild-type and each of the PAI-1 parental mutants in cell-free extracts. The inhibitory activity is expressed as the percentage of active PAI-1 protein relative to the total soluble protein of the cell extracts.
Rabbit Anti Human Upa Antibody 389, supplied by American Diagnostica, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Innovative Research Inc rabbit anti human urokinase (upa) polyclonal fractionated,rabbit anti-human upa igg
FIG. 1. Screen of 33B8 binding deficient PAI-1 mutants. Panels A–C, filter series showing EX(lox) plaques. Panel A, plaques immunore- active with murine monoclonal 33B8; panel B, plaques reactive with rabbit anti-PAI-1 polyclonal antibodies; panel C, a merged image of A and B. The same filter is shown in each case. The dark spots indicate PAI-1antibody complexes, and the circles indicate examples of PAI-1 mutants that are specifically disrupted in 33B8 binding. Panels D and E show immunoblots of cell-free extracts of wild-type and parental PAI-1 mutants selected from the screen. Panel D was probed with 33B8 and panel E with anti-PAI-1 polyclonal antibodies. Panel F shows the inhibitory activity toward <t>high</t> <t>molecular</t> weight <t>uPA</t> of wild-type and each of the PAI-1 parental mutants in cell-free extracts. The inhibitory activity is expressed as the percentage of active PAI-1 protein relative to the total soluble protein of the cell extracts.
Rabbit Anti Human Urokinase (Upa) Polyclonal Fractionated,Rabbit Anti Human Upa Igg, supplied by Innovative Research Inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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FIG. 1. Screen of 33B8 binding deficient PAI-1 mutants. Panels A–C, filter series showing EX(lox) plaques. Panel A, plaques immunore- active with murine monoclonal 33B8; panel B, plaques reactive with rabbit anti-PAI-1 polyclonal antibodies; panel C, a merged image of A and B. The same filter is shown in each case. The dark spots indicate PAI-1antibody complexes, and the circles indicate examples of PAI-1 mutants that are specifically disrupted in 33B8 binding. Panels D and E show immunoblots of cell-free extracts of wild-type and parental PAI-1 mutants selected from the screen. Panel D was probed with 33B8 and panel E with anti-PAI-1 polyclonal antibodies. Panel F shows the inhibitory activity toward <t>high</t> <t>molecular</t> weight <t>uPA</t> of wild-type and each of the PAI-1 parental mutants in cell-free extracts. The inhibitory activity is expressed as the percentage of active PAI-1 protein relative to the total soluble protein of the cell extracts.
Biotin Labeled Human Upa, supplied by Innovative Research Inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Huabio Inc rabbit anti-human upa
FIG. 1. Screen of 33B8 binding deficient PAI-1 mutants. Panels A–C, filter series showing EX(lox) plaques. Panel A, plaques immunore- active with murine monoclonal 33B8; panel B, plaques reactive with rabbit anti-PAI-1 polyclonal antibodies; panel C, a merged image of A and B. The same filter is shown in each case. The dark spots indicate PAI-1antibody complexes, and the circles indicate examples of PAI-1 mutants that are specifically disrupted in 33B8 binding. Panels D and E show immunoblots of cell-free extracts of wild-type and parental PAI-1 mutants selected from the screen. Panel D was probed with 33B8 and panel E with anti-PAI-1 polyclonal antibodies. Panel F shows the inhibitory activity toward <t>high</t> <t>molecular</t> weight <t>uPA</t> of wild-type and each of the PAI-1 parental mutants in cell-free extracts. The inhibitory activity is expressed as the percentage of active PAI-1 protein relative to the total soluble protein of the cell extracts.
Rabbit Anti Human Upa, supplied by Huabio Inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Image Search Results


FIG. 1. Screen of 33B8 binding deficient PAI-1 mutants. Panels A–C, filter series showing EX(lox) plaques. Panel A, plaques immunore- active with murine monoclonal 33B8; panel B, plaques reactive with rabbit anti-PAI-1 polyclonal antibodies; panel C, a merged image of A and B. The same filter is shown in each case. The dark spots indicate PAI-1antibody complexes, and the circles indicate examples of PAI-1 mutants that are specifically disrupted in 33B8 binding. Panels D and E show immunoblots of cell-free extracts of wild-type and parental PAI-1 mutants selected from the screen. Panel D was probed with 33B8 and panel E with anti-PAI-1 polyclonal antibodies. Panel F shows the inhibitory activity toward high molecular weight uPA of wild-type and each of the PAI-1 parental mutants in cell-free extracts. The inhibitory activity is expressed as the percentage of active PAI-1 protein relative to the total soluble protein of the cell extracts.

Journal: Journal of Biological Chemistry

Article Title: Mapping of a Conformational Epitope on Plasminogen Activator Inhibitor-1 by Random Mutagenesis

doi: 10.1074/jbc.m208420200

Figure Lengend Snippet: FIG. 1. Screen of 33B8 binding deficient PAI-1 mutants. Panels A–C, filter series showing EX(lox) plaques. Panel A, plaques immunore- active with murine monoclonal 33B8; panel B, plaques reactive with rabbit anti-PAI-1 polyclonal antibodies; panel C, a merged image of A and B. The same filter is shown in each case. The dark spots indicate PAI-1antibody complexes, and the circles indicate examples of PAI-1 mutants that are specifically disrupted in 33B8 binding. Panels D and E show immunoblots of cell-free extracts of wild-type and parental PAI-1 mutants selected from the screen. Panel D was probed with 33B8 and panel E with anti-PAI-1 polyclonal antibodies. Panel F shows the inhibitory activity toward high molecular weight uPA of wild-type and each of the PAI-1 parental mutants in cell-free extracts. The inhibitory activity is expressed as the percentage of active PAI-1 protein relative to the total soluble protein of the cell extracts.

Article Snippet: Materials—Murine monoclonal antibodies MA-33B8 (33B8) and MA31C9 (31C9) as well as rabbit polyclonal antibodies directed against human PAI-1 were purchased from Molecular Innovations (Southfield, MI), horseradish peroxidase-conjugated goat anti-mouse and anti-rabbit antiserum were from Bio-Rad. tPA was from Genentech (South San Francisco, CA), and uPA was from Molecular Innovations.

Techniques: Binding Assay, Western Blot, Activity Assay, High Molecular Weight

FIG. 4. Analysis of site-directed PAI-1 mutants. Panels A and B show immunoblots of cell-free extracts of wild-type and site-directed PAI-1 mutants. Panel A was probed with 33B8 and panel B with anti-PAI-1 polyclonal antibodies. Panel C shows the inhibitory activity toward high molecular weight uPA of wild-type and each of the PAI-1 site-directed mutants in cell-free extracts. The inhibitory activity is expressed as the percentage of functionally active PAI-1 relative to the total soluble protein of the cell extracts.

Journal: Journal of Biological Chemistry

Article Title: Mapping of a Conformational Epitope on Plasminogen Activator Inhibitor-1 by Random Mutagenesis

doi: 10.1074/jbc.m208420200

Figure Lengend Snippet: FIG. 4. Analysis of site-directed PAI-1 mutants. Panels A and B show immunoblots of cell-free extracts of wild-type and site-directed PAI-1 mutants. Panel A was probed with 33B8 and panel B with anti-PAI-1 polyclonal antibodies. Panel C shows the inhibitory activity toward high molecular weight uPA of wild-type and each of the PAI-1 site-directed mutants in cell-free extracts. The inhibitory activity is expressed as the percentage of functionally active PAI-1 relative to the total soluble protein of the cell extracts.

Article Snippet: Materials—Murine monoclonal antibodies MA-33B8 (33B8) and MA31C9 (31C9) as well as rabbit polyclonal antibodies directed against human PAI-1 were purchased from Molecular Innovations (Southfield, MI), horseradish peroxidase-conjugated goat anti-mouse and anti-rabbit antiserum were from Bio-Rad. tPA was from Genentech (South San Francisco, CA), and uPA was from Molecular Innovations.

Techniques: Western Blot, Activity Assay, High Molecular Weight